2GBM

Crystal Structure of the 35-36 8 Glycine Insertion Mutant of Ubiquitin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-24
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	48.817, 49.786, 123.147
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.511 - 1.550
R-factor	0.213
Rwork	0.213
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ubq
RMSD bond length	0.013
RMSD bond angle	1.391
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.511	1.600
High resolution limit [Å]	1.550	1.550
Rmerge	0.054	0.179
Number of reflections	44135
<I/σ(I)>	20.02	10.7
Completeness [%]	98.5	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5	293.15	25-30% PEG 4000, 50 mM Sodium Cacodylate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K, pH 5.50