2GBJ
Crystal Structure of the 9-10 8 Glycine Insertion Mutant of Ubiquitin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-17 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.12711 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.124, 53.035, 87.268 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.691 - 1.350 |
| R-factor | 0.18998 |
| Rwork | 0.188 |
| R-free | 0.22589 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.416 |
| Data scaling software | d*TREK (9.5LDz) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.691 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.068 | 0.503 |
| Total number of observations | 10991 | |
| Number of reflections | 27867 | 2511 |
| <I/σ(I)> | 10.8 | 2.4 |
| Completeness [%] | 97.8 | 90.6 |
| Redundancy | 4.98 | 4.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 279.15 | 27-32% PEG 4000, 50 mM Sodium Cacodylate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 279.15K |
