2GB0
Monomeric sarcosine oxidase: structure of a covalently flavinylated amine oxidizing enzyme
Replaces: 1L9FReplaces: 1B3MExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-08 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 0.9792, 0.9794, 0.9392, 1.0679 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.562, 69.555, 74.117 |
| Unit cell angles | 90.00, 94.26, 90.00 |
Refinement procedure
| Resolution | 42.530 - 1.850 |
| Rwork | 0.165 |
| R-free | 0.19800 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.078 | 0.296 |
| Number of reflections | 58976 | |
| <I/σ(I)> | 14.6 | 1.5 |
| Completeness [%] | 94.0 | 57.6 |
| Redundancy | 3.1 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 298 | PHOSPHATE, VAPOR DIFFUSION, SITTING DROP AT 298K, pH 6.70 |
