2GAO
Crystal Structure of Human SAR1a in Complex With GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-03-07 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.295, 63.814, 62.132 |
| Unit cell angles | 90.00, 105.94, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| Rwork | 0.224 |
| R-free | 0.26940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1F6B |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.422 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.199 | 0.057 | |
| Number of reflections | 29052 | 2990 | 2875 |
| <I/σ(I)> | 5 | ||
| Completeness [%] | 99.9 | ||
| Redundancy | 4.3 | 4.3 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 30% PEG8000, 0.2M ammonium sulfate, vapor diffusion, sitting drop, temperature 291K |
