2GAN
Crystal Structure of a Putative Acetyltransferase from Pyrococcus horikoshii, Northeast Structural Genomics Target JR32.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-22 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97904 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.706, 76.660, 45.005 |
| Unit cell angles | 90.00, 100.32, 90.00 |
Refinement procedure
| Resolution | 24.410 - 2.100 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.25400 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.410 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 0.340 |
| Number of reflections | 49028 | |
| <I/σ(I)> | 14.72 | 3.85 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 4.1 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 10 mM Tris (pH 7.5), 16% PEG3350, 350 mM ammonium sulfate, and 5 mM DTT., VAPOR DIFFUSION, HANGING DROP, temperature 291K |
