2GAH
Heterotetrameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-03 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.259, 132.772, 198.038 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.570 - 2.000 |
| Rwork | 0.174 |
| R-free | 0.21100 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.097 | 0.446 |
| Number of reflections | 103177 | |
| <I/σ(I)> | 16.9 | 3.4 |
| Completeness [%] | 89.5 | 87 |
| Redundancy | 8.8 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | 18% PEG 20000, 0.1M Tris, 0.04M sodium fuorate and 0.1M sodium chloride., pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
