2G9Z
Thiamin pyrophosphokinase from Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.825, 60.331, 63.722 |
| Unit cell angles | 66.14, 89.94, 65.07 |
Refinement procedure
| Resolution | 29.440 - 1.960 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ig0 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.440 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.035 | 0.195 |
| Number of reflections | 40488 | |
| <I/σ(I)> | 14.5 | 3.8 |
| Completeness [%] | 91.8 | 60.3 |
| Redundancy | 1.9 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 17.5% PEG4000, 0.2M Magnesium chloride, 0.1M Tris, 10% Glycerol, Protein incubated with Thiamin and AMP-PNP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
