2G9N
Structure of the DEAD domain of Human eukaryotic initiation factor 4A, eIF4A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Detector technology | CCD |
| Collection date | 2006-02-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.93300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.799, 78.252, 59.088 |
| Unit cell angles | 90.00, 103.43, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.250 |
| R-factor | 0.18026 |
| Rwork | 0.176 |
| R-free | 0.25730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qde |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.732 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.370 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 19100 | |
| <I/σ(I)> | 7.4 | 5.3 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.45 | 3.46 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% PEG3350, 200 mM Ammonium Nitrate, 500 mM NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
