2G9E
Protonation-mediated structural flexibility in the F conjugation regulatory protein, TRAM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 105 |
Detector technology | CCD |
Collection date | 2005-11-08 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.11587 |
Spacegroup name | I 4 |
Unit cell lengths | 50.939, 50.939, 46.551 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.010 - 1.800 |
R-factor | 0.18511 |
Rwork | 0.182 |
R-free | 0.24459 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g7o |
RMSD bond length | 0.012 |
RMSD bond angle | 1.193 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.010 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.045 | 0.362 |
Number of reflections | 5552 | |
<I/σ(I)> | 25.1 | 2.38 |
Completeness [%] | 98.6 | 84.1 |
Redundancy | 3.6 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 30% PEG 400, 0.1M MES PH 6.5, 2MM DTT, 0.5M AMMONIUM ACETATE , pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 295K |