2G8V
B. halodurans RNase H catalytic domain E188A mutant in complex with Mg2+ and RNA/DNA hybrid (reaction product)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-03-10 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.871, 36.809, 62.144 |
| Unit cell angles | 90.00, 97.17, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| Rwork | 0.198 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1zbi |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.130 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 14810 | |
| Completeness [%] | 92.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 278 | 20% EtOH, 0.1 M Tris pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | EtOH | ||
| 2 | 1 | 1 | Tris | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | EtOH | ||
| 5 | 1 | 2 | Tris |
