2G80

Crystal structure of UTR4 protein (Unknown transcript 4 protein) (yel038w) from Saccharomyces cerevisiae at 2.28 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-09-26
Detector	ADSC QUANTUM 210
Spacegroup name	P 1 21 1
Unit cell lengths	74.688, 102.016, 77.227
Unit cell angles	90.00, 92.68, 90.00

Refinement procedure

Resolution	61.530 - 2.280
R-factor	0.18
Rwork	0.177
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zs9A
RMSD bond length	0.016
RMSD bond angle	1.604
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	61.540	102.060	2.400
High resolution limit [Å]	2.280	7.210	2.280
Rmerge	0.088	0.046	0.359
Total number of observations		5849	24462
Number of reflections	52775	1727	7673
<I/σ(I)>	4.5	12.8	1.7
Completeness [%]	99.9	99.5	99.9
Redundancy	3.6	3.4	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP, NANODROP		290	21.00% PEG 3350, 0.10M NP_Magnesium Nitrate, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 290K