2G76
Crystal structure of human 3-phosphoglycerate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99806 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.084, 124.113, 59.502 |
| Unit cell angles | 90.00, 100.99, 90.00 |
Refinement procedure
| Resolution | 36.200 - 1.700 |
| R-factor | 0.1828 |
| Rwork | 0.181 |
| R-free | 0.21644 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ygy 1psd |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.453 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.200 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 66626 | |
| Completeness [%] | 98.9 | 96.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M MMT, 30 % PEG1k, 5mM NAD+, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
