2G43

Structure of the ZNF UBP domain from deubiquitinating enzyme isopeptidase T (IsoT)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-05
Detector	ADSC QUANTUM 4
Wavelength(s)	1.28020, 1.2790, 1.12810 0.97623
Spacegroup name	C 1 2 1
Unit cell lengths	61.380, 86.180, 59.900
Unit cell angles	90.00, 99.29, 90.00

Refinement procedure

Resolution	35.000 - 2.090
R-factor	0.228
Rwork	0.228
R-free	0.27400
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.400
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.160
High resolution limit [Å]	2.090	2.090
Rmerge	0.047	0.064
Number of reflections	17788
<I/σ(I)>	27.7	16.7
Completeness [%]	96.7	97.2
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	300	16% polyethylene glycol 8000, 0.8 M sodium cacodylate pH 6.5, 0.16 M calcium acetate, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 300K, pH 6.50
1		6.5	300	16% polyethylene glycol 8000, 0.8 M sodium cacodylate pH 6.5, 0.16 M calcium acetate, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 300K, pH 6.50