2G42
Crystal structure of a putative nucleic acid binding protein (tm0693) from thermotoga maritima at 2.28 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-15 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.174, 26.761, 73.221 |
| Unit cell angles | 90.00, 97.23, 90.00 |
Refinement procedure
| Resolution | 40.800 - 2.280 |
| R-factor | 0.206 |
| Rwork | 0.203 |
| R-free | 0.25100 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.920 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.859 | 2.350 | |
| High resolution limit [Å] | 2.280 | 3.870 | 2.280 |
| Rmerge | 0.091 | 0.055 | 0.475 |
| Number of reflections | 7150 | 766 | 385 |
| <I/σ(I)> | 12.72 | 25.8 | 2.73 |
| Completeness [%] | 94.9 | 98.7 | 66.4 |
| Redundancy | 6.777 | 3.398 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 5.1 | 277 | 0.2M CaCl2, 20.0% PEG-3350, No Buffer, pH 5.1, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |
