2G3O
The 2.1A crystal structure of copGFP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 |
| Unit cell lengths | 145.954, 145.954, 53.767 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.710 - 2.100 |
| R-factor | 0.2325 |
| Rwork | 0.230 |
| R-free | 0.27160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.723 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.714 | 2.155 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 74388 | |
| Completeness [%] | 99.5 | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 22% PEG 3350, 0.1M Sodium Acetate, 0.15M Magnesium Sulphate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
