2G2I
A Src-like Inactive Conformation in the Abl Tyrosine Kinase Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 88.457, 88.457, 235.625 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.120 |
| R-factor | 0.251 |
| Rwork | 0.249 |
| R-free | 0.30800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m52 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.670 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.120 |
| Rmerge | 0.130 | 0.445 |
| Number of reflections | 17209 | |
| <I/σ(I)> | 14.2 | 2.75 |
| Completeness [%] | 99.4 | 95.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | 2M sodium malonate , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
