2G2C
Putative molybdenum cofactor biosynthesis protein from Corynebacterium diphtheriae.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-11-09 |
Detector | SBC-3 |
Wavelength(s) | 0.97894 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 67.822, 67.822, 114.276 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.520 - 1.500 |
R-factor | 0.1739 |
Rwork | 0.171 |
R-free | 0.20210 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.535 |
Data scaling software | SCALEPACK |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.510 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.073 | 0.664 |
Number of reflections | 23460 | |
<I/σ(I)> | 49.3 | 2.23 |
Completeness [%] | 91.4 | 49.5 |
Redundancy | 29 | 8.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 0.1 M Hepes buffer, 3 M NaCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |