2G0T
Crystal structure of a putative nucleotide binding protein (tm0796) from Thermotoga maritima at 2.67 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-06 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979224, 0.979508, 0.885567 |
Spacegroup name | P 41 3 2 |
Unit cell lengths | 191.634, 191.634, 191.634 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.570 - 2.670 |
R-factor | 0.208 |
Rwork | 0.207 |
R-free | 0.23800 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 2.367 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.928 | 29.930 | 2.740 |
High resolution limit [Å] | 2.670 | 11.940 | 2.670 |
Rmerge | 0.188 | 0.059 | 0.892 |
Total number of observations | 3805 | 14093 | |
Number of reflections | 34565 | 442 | 2426 |
<I/σ(I)> | 3.7 | 10.8 | 0.8 |
Completeness [%] | 99.6 | 93.7 | 96.5 |
Redundancy | 9.5 | 8.6 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP, NANODROP | 6.6 | 277 | 0.2M NH4Formate, 20.0% PEG-3350, No Buffer, pH 6.6, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K |