2G0F
Crystal Structure of P144A mutant of E.coli CcmG protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | CONVENTIONAL Cu |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2005-12-24 |
Detector | BRUKER APEX |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.416, 47.783, 85.432 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.720 - 2.200 |
R-factor | 0.186 |
Rwork | 0.186 |
R-free | 0.23800 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 2b1k |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | SMART |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.104 | 0.594 |
Number of reflections | 7932 | |
Completeness [%] | 99.8 | 98.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 2.0M ammonium sulfate, 0.1M HEPES buffer (pH=8.0),2%(w/v) PEG 4K, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |