2FZP
Crystal structure of the USP8 interaction domain of human NRDP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-04 |
| Detector | SBC-3 |
| Wavelength(s) | 1.00769 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.796, 44.262, 88.339 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.360 - 1.870 |
| R-factor | 0.172 |
| Rwork | 0.169 |
| R-free | 0.23163 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.552 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.360 | 1.940 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.140 | 0.653 |
| Number of reflections | 12100 | |
| <I/σ(I)> | 8.97 | 1.52 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 4.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 1.8M (NH4)2SO4, 0.1M HEPES pH 7.0,0.2M NaCl, 0.001M DTT, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
