2FZL
Structure of C-terminal domain of Archaeoglobus fulgidus XPB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.115869 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 172.859, 172.859, 172.859 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.2481 |
| Rwork | 0.249 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fwr |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.068 | 0.629 |
| Number of reflections | 10024 | 978 |
| <I/σ(I)> | 55.18 | 5.06 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 25.5 | 25.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.8 | 303 | Ammonium sulphate, isopropanol, CAPS, pH 9.8, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
