2FYX

Crystal structure of a putative transposase (dr_0177) from deinococcus radiodurans r1 at 1.90 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2004-08-25
Detector	ADSC QUANTUM 4
Spacegroup name	P 32
Unit cell lengths	66.787, 66.787, 81.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.240 - 1.900
R-factor	0.16159
Rwork	0.160
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2f4f chain A
RMSD bond length	0.017
RMSD bond angle	1.703
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.915	28.920	1.950
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.045	0.029	0.547
Total number of observations		1585	6798
Number of reflections	31895	358	2360
<I/σ(I)>	9.9	19.7	1.3
Completeness [%]	100.0
Redundancy	3.9	4.4	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	6.5	277	2.0M (NH4)2SO4, 0.2M NaCl, 0.1M Cacodylate pH 6.5, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K