2FYS
Crystal structure of Erk2 complex with KIM peptide derived from MKP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-04-14 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.377, 67.478, 86.605 |
| Unit cell angles | 90.00, 99.55, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| Rwork | 0.174 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2erk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.087 |
| Number of reflections | 20430 |
| Completeness [%] | 89.8 |
| Redundancy | 3.12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | 20% PEG3350, 0.1~0.2 M ammonium chloride, 0.1M 2- morpholinoethanesulfonic acid (pH 6.5)., VAPOR DIFFUSION, HANGING DROP, temperature 294K |
