2FYQ

Crystal Structure of the Norwalk Virus Protease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	93
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9794
Spacegroup name	P 65 2 2
Unit cell lengths	121.833, 121.833, 51.369
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	105.410 - 1.500
Rwork	0.185
R-free	0.21100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.207
Data scaling software	d*TREK (9.4LDz)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.500	1.550
High resolution limit [Å]	1.500	1.500
Rmerge		0.457
Number of reflections	36055
<I/σ(I)>		4.1
Completeness [%]	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5	298	0.1M lithium sulfate, 0.1M bis-tris, 0.1M EDTA, 20% PEG 3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 5.50