2FYG
Crystal structure of NSP10 from Sars coronavirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2005-09-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 108.874, 108.874, 108.874 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.460 - 1.800 |
| R-factor | 0.19764 |
| Rwork | 0.196 |
| R-free | 0.23295 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.488 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.460 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.048 | 0.600 |
| Number of reflections | 19037 | |
| <I/σ(I)> | 2.36 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.7 | 3.14 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 2.0 M AMMONIUM FORMATE, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
