2FXL

Urate oxidase from aspergillus flavus complexed with allantoin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	277
Detector technology	CCD
Collection date	2005-12-05
Detector	MARRESEARCH
Wavelength(s)	0.962
Spacegroup name	I 2 2 2
Unit cell lengths	80.364, 96.086, 105.368
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.760
R-factor	0.1906
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1r4s
RMSD bond length	0.036
RMSD bond angle	0.079
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	SHELXL

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.900	1.850
High resolution limit [Å]	1.760	1.760
Rmerge	0.070
Number of reflections	33267
<I/σ(I)>	18.7	5.1
Completeness [%]	98.1	97.6
Redundancy	4.8	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	290	CRYSTALLIZATION CONDITIONS: 8.5MG/ML PROTEIN, EXCESS URIC ACID, 5-7% W/V PEG 8000, 100MM TRIS/HCL, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 291K, VAPOR DIFFUSION, HANGING DROP, temperature 290K