2FXF
Human spermidine/spermine N1-acetyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-02-02 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 65.631, 66.738, 95.660 |
| Unit cell angles | 90.00, 94.41, 90.00 |
Refinement procedure
| Resolution | 32.720 - 2.000 |
| R-factor | 0.19635 |
| Rwork | 0.194 |
| R-free | 0.24993 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 2F5I |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.766 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 27544 | |
| Completeness [%] | 98.9 | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 300 | 14% PEG3350, 0.2 M Ca Acetate, 0.1 M BisTris propane, pH 7.0. , VAPOR DIFFUSION, HANGING DROP, temperature 300K |
