2FWM
Crystal Structure of E. coli EntA, a 2,3-dihydrodihydroxy benzoate dehydrogenase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 113 |
| Detector technology | CCD |
| Collection date | 2005-08-02 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 58.585, 66.643, 109.732 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.000 |
| Rwork | 0.186 |
| R-free | 0.21400 |
| Structure solution method | MAD |
| RMSD bond length | 1569.000 |
| RMSD bond angle | 2129.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.059 | 0.298 |
| Number of reflections | 14485 | |
| Completeness [%] | 95.4 | 70 |
| Redundancy | 4.3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 277 | 2% PEG4000, 2% ethylene glycol, 300 mM AmSO4, 50 mM NaSuccinate, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 5.00 |
