2FW0
Apo Open Form of Glucose/Galactose Binding Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-14 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.800, 70.300, 112.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 1.550 |
| R-factor | 0.165 |
| Rwork | 0.165 |
| R-free | 0.17800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 2FVY |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.200 |
| Data reduction software | SAINT |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.039 | 0.180 |
| Number of reflections | 65759 | |
| <I/σ(I)> | 8.31 | |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 50mM NaCitrate, 2.5M AmSO4, 2.0 M Malonate cryoprotectant, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
