2FVK
Crystal structure of dihydropyrimidinase from Saccharomyces kluyveri in complex with the substrate dihydrouracil
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.130, 71.600, 161.890 |
| Unit cell angles | 90.00, 91.40, 90.00 |
Refinement procedure
| Resolution | 54.230 - 2.400 |
| R-factor | 0.18064 |
| Rwork | 0.178 |
| R-free | 0.23352 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2fty |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.179 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.230 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 80380 | |
| <I/σ(I)> | 11 | 2.3 |
| Completeness [%] | 99.2 | 95.7 |
| Redundancy | 2.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 3350, 0.1M ammonium sulfate, 1mM CaCl2, 0.1M bis-tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
