2FV9

Crystal structure of TACE in complex with JMV 390-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2002-12-15
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	73.799, 76.221, 102.092
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.020
R-factor	0.253
Rwork	0.251
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 1BKC
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.090
High resolution limit [Å]	2.020	2.020
Rmerge	0.047	0.513
Number of reflections	35008	3461
<I/σ(I)>	16.7
Completeness [%]	90.9	91.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.6	295	15% PEG 6k, 10% 2-Propanol, 100 mM sodium citrate, pH 5.6, VAPOR DIFFUSION, temperature 295K