2FUA

L-FUCULOSE 1-PHOSPHATE ALDOLASE CRYSTAL FORM T WITH COBALT

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1994
Detector	SIEMENS-NICOLET X100
Spacegroup name	P 4 21 2
Unit cell lengths	93.700, 93.700, 42.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.18
Rwork	0.180
RMSD bond length	0.013
RMSD bond angle	1.700
Data reduction software	XDS
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.000 *	2.000 *
Rmerge	0.061	0.159 *
Number of reflections	15207
Completeness [%]	89.0 *	80 *
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.6 *	293 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop		0.5 (mM)
4	1	drop	beta-mercaptoethanol	10 (mM)
5	1	reservoir	ammonium sulfate	1.62 (M)
6	1	reservoir	potassium phosphate	20 (mM)
7	1	reservoir		4 (mM)
8	1	reservoir	beta-mercaptoethanol	4 (mM)