2FTK
berylloflouride Spo0F complex with Spo0B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2005-07-14 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.467, 118.331, 168.235 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.860 - 3.050 |
Rwork | 0.232 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f51 |
RMSD bond length | 0.010 |
RMSD bond angle | 0.009 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.860 | 3.240 |
High resolution limit [Å] | 3.050 | 3.050 |
Number of reflections | 28073 | |
Completeness [%] | 98.6 | 90.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.1 | 297 | 0.5 M KCl, 25% PEG2000, 100mM Tris HCl ph8.1, VAPOR DIFFUSION, temperature 297K |