2FT8
Structure of Cu(I)azurin, pH8, with the metal-binding loop sequence "CTFPGHSALM" replaced with "CTPHPM"
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX10.1 |
Synchrotron site | SRS |
Beamline | PX10.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-16 |
Detector | MARRESEARCH |
Wavelength(s) | 1.075 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 51.953, 51.953, 75.434 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.800 - 1.550 |
R-factor | 0.142 |
Rwork | 0.139 |
R-free | 0.19100 |
Structure solution method | Direct refinement from refined AZAMI-F Cu(II) structure |
RMSD bond length | 0.017 |
RMSD bond angle | 1.609 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.800 | 1.630 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.064 | 0.358 |
Total number of observations | 17358 | |
Number of reflections | 15561 | 2162 |
<I/σ(I)> | 7.3 | 2.2 |
Completeness [%] | 99.5 | 97.7 |
Redundancy | 8.3 | 8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1MM MES, 20% PEG6000, 0.2M LiCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |