2FSY

Bacteriophage HK97 Pepsin-treated Expansion Intermediate IV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-31
Detector	ADSC QUANTUM 315
Wavelength(s)	1.181
Spacegroup name	P 43 21 2
Unit cell lengths	1006.394, 1006.394, 728.693
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.800
Rwork	0.337
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ohg
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.870
High resolution limit [Å]	3.800	3.800
Rmerge	0.170	0.425
Number of reflections	2144154	48089
<I/σ(I)>	5.5	1.8
Completeness [%]	59.9	44.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	100mM Sodium Citrate pH 3.5, 400mM Magnesium Acetate Hexahydrate, 25% 1,6-Hexanediol, pH 5.2, vapor diffusion, hanging drop, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	100mM Sodium Citrate pH 3.5, 400mM Magnesium Acetate Hexahydrate, 25% 1,6-Hexanediol, pH 5.2, vapor diffusion, hanging drop, temperature 293K