2FST

mitogen activated protein kinase p38alpha (D176A+F327L) activating mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2005-06-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.925
Spacegroup name	P 21 21 21
Unit cell lengths	68.673, 69.786, 74.227
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.450
R-factor	0.20791
Rwork	0.206
R-free	0.24062
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.467
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.500
High resolution limit [Å]	1.450	1.450
Number of reflections	63600
<I/σ(I)>	46.1	3.1
Completeness [%]	99.4	100
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	277	18% PEG 3350, 0.2M KF, 0.1 HEPES, 25mM bOG, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 277K