2FSR
Crystal Structure of the Acetyltransferase from Agrobacterium tumefaciens str. C58
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97912 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 89.468, 106.778, 42.099 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.880 - 1.520 |
| R-factor | 0.18147 |
| Rwork | 0.179 |
| R-free | 0.19935 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.220 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.880 | 1.570 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.081 | 0.320 |
| Number of reflections | 30447 | |
| <I/σ(I)> | 12.8 | 4.97 |
| Completeness [%] | 96.8 | 81.4 |
| Redundancy | 12.9 | 8.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.1M Bis-tris, 0.2M NH4(OAC), 25% PEG3350, 4% Jeffamine M-600, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
