2FSJ
Crystal structure of Ta0583, an archaeal actin homolog, native data
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.95436 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.265, 50.762, 123.391 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.695 - 1.900 |
| Rwork | 0.196 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.600 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.695 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.078 | 0.292 |
| Total number of observations | 9580 | |
| Number of reflections | 26096 | 3666 |
| <I/σ(I)> | 6.4 | 2.2 |
| Completeness [%] | 99.7 | 98.6 |
| Redundancy | 3.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM Bis-Tris pH 6.5, 15-17% PEG-2000MME, 21-22% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
