2FS7
Crystal Structure of Apo-Cellular Retinoic Acid Binding Protein Type II At 1.55 Angstroms Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2005-04-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 34.475, 37.220, 57.597 |
| Unit cell angles | 73.09, 75.74, 87.22 |
Refinement procedure
| Resolution | 50.000 - 1.550 |
| R-factor | 0.149 |
| Rwork | 0.144 |
| R-free | 0.20100 |
| Structure solution method | Rigid Body Refinement |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.742 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.042 | 0.157 |
| Number of reflections | 35956 | 2650 |
| <I/σ(I)> | 23.7 | 3.36 |
| Completeness [%] | 92.3 | 68.1 |
| Redundancy | 2.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 30% (w/v) PEG 8000, 0.1 M Tris-HCl pH 8.0, and 0.2 M Sodium Acetate, vapor diffusion, hanging drop, temperature 298K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 30% (w/v) PEG 8000, 0.1 M Tris-HCl pH 8.0, and 0.2 M Sodium Acetate, vapor diffusion, hanging drop, temperature 298K |
