2FRX
Crystal structure of YebU, a m5C RNA methyltransferase from E.coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-19 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.962 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.706, 87.126, 95.048 |
| Unit cell angles | 88.33, 76.79, 90.19 |
Refinement procedure
| Resolution | 29.030 - 2.900 |
| R-factor | 0.234 |
| Rwork | 0.231 |
| R-free | 0.28200 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.758 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 45870 | |
| <I/σ(I)> | 9.13 | 2.14 |
| Completeness [%] | 97.9 | 97.5 |
| Redundancy | 3.22 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 15%(w/v) PEG 8000, 50mM mono-potassium dihydrogen phosphate, 10mM BaCl2 or 15% PEG 5000 MME, sodium acetate pH 4.6, 0.2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
