2FRH
Crystal Structure of Sara, A Transcription Regulator From Staphylococcus Aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2001-09-01 |
| Detector | PRINCETON 2K |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 65.338, 65.338, 237.289 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.500 - 2.500 |
| R-factor | 0.266 |
| Rwork | 0.266 |
| R-free | 0.30200 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.072 |
| Number of reflections | 10107 |
| <I/σ(I)> | 33 |
| Completeness [%] | 89.7 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | PEG 4000, PH 6.5, VAPOR DIFFUSION, HANGING DROP AT 277K, pH 6.50 |
