2FR5
Crystal Structure of Mouse Cytidine Deaminase Complexed with Tetrahydrouridine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-05-23 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 82.238, 93.403, 180.743 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.490 - 1.480 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mq0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.447 |
| Data reduction software | BBS |
| Data scaling software | d*TREK (8.0SSI) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.490 | 1.530 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.073 | 0.288 |
| Total number of observations | 73184 | |
| Number of reflections | 111799 | 11283 |
| <I/σ(I)> | 15.6 | 5 |
| Completeness [%] | 96.7 | 98.9 |
| Redundancy | 6.63 | 5.26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 0.8M AMMONIUM SULPHATE, 0.1M CITRATE, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
