Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-16 |
| Detector | ENRAF-NONIUS |
| Spacegroup name | P 61 |
| Unit cell lengths | 83.454, 83.454, 106.825 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.202 |
| Rwork | 0.197 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pzn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.230 |
| Data reduction software | LSCALE |
| Data scaling software | LSCALE |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.046 | 0.244 |
| Number of reflections | 17245 | |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 91.2 | 89.5 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 294 | 7% PEG 3350, 50 MM HEPES BUFFER, 50 MM MGCL2, 0.6 M K AC, pH 7.40, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
