2FPH
Cell division protein ylmH from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-17 |
| Detector | MACSCIENCE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.180, 46.267, 50.261 |
| Unit cell angles | 90.00, 99.70, 90.00 |
Refinement procedure
| Resolution | 49.390 - 1.700 |
| R-factor | 0.243 |
| Rwork | 0.240 |
| R-free | 0.29900 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.155 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.068 | 0.217 |
| Number of reflections | 17137 | |
| <I/σ(I)> | 5.2 | 2.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 293 | 10% PEG6000, 1.0M LiCl, 100mM citrate buffer, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 5.00 |
