2FPB
Structure of Strictosidine Synthase, the Biosynthetic Entry to the Monoterpenoid Indole Alkaloid Family
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-14 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9714 |
| Spacegroup name | H 3 |
| Unit cell lengths | 148.936, 148.936, 121.344 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.16631 |
| Rwork | 0.164 |
| R-free | 0.21717 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.707 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.840 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.099 | 0.436 |
| Number of reflections | 23380 | |
| <I/σ(I)> | 3 | 3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 295 | 0.8M potassium sodium tartrate tetrahydrate, 100mM HEPES-Na, 1mM Tryptamine, pH 7.5, VAPOR DIFFUSION, temperature 295.0K |
