2FP9
Crystal structure of Native Strictosidine Synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-03-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9330 |
| Spacegroup name | H 3 |
| Unit cell lengths | 150.276, 150.276, 122.399 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.960 |
| R-factor | 0.19156 |
| Rwork | 0.189 |
| R-free | 0.23658 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.995 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.990 |
| High resolution limit [Å] | 2.960 | 2.960 |
| Rmerge | 0.074 | |
| Number of reflections | 20014 | |
| <I/σ(I)> | 1.9 | |
| Completeness [%] | 98.4 | 99.3 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 295 | 0.8M potassium sodium tartrate tetrahydrate, 100mM HEPES-Na, pH 7.5, VAPOR DIFFUSION, temperature 295.0K |
