2FON
X-ray crystal structure of LeACX1, an acyl-CoA oxidase from Lycopersicon esculentum (tomato)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 32-ID |
Synchrotron site | APS |
Beamline | 32-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-28 |
Detector | MARRESEARCH |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 120.200, 240.330, 89.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.740 |
Rwork | 0.212 |
R-free | 0.26700 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.863 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.770 |
High resolution limit [Å] | 2.700 | 6.490 | 2.700 |
Rmerge | 0.092 | 0.078 | 0.374 |
Number of reflections | 64138 | 5105 | 4704 |
<I/σ(I)> | 12.2 | ||
Completeness [%] | 95.4 | ||
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | 10% 2-methyl-2,4-pentanediol, 7% PEG 6000, 100 mM Tris, 1 mM acetyl-CoA, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | 10% 2-methyl-2,4-pentanediol, 7% PEG 6000, 100 mM Tris, 1 mM acetyl-CoA, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |