2FOL

Crystal structure of human RAB1A in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-01-09
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	75.790, 75.790, 49.080
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.631
Rwork	0.199
R-free	0.25230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdb entries 1YZN 1ukv
RMSD bond length	0.019
RMSD bond angle	1.397
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (refmac_5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.710
High resolution limit [Å]	2.620	5.630	2.620
Rmerge	0.088	0.034	0.826
Number of reflections	5102
Completeness [%]	100.0
Redundancy	10.6	10	10.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	25% PEG3350, 0.2M magnesium chloride, 0.1M Tris, pH 8.5, vapor diffusion, sitting drop, temperature 291K