2FNW
Pseudomonas aeruginosa E2Q/H83Q/M109H-azurin RE(PHEN)(CO)3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.945 |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.935, 38.334, 48.835 |
| Unit cell angles | 100.50, 106.25, 109.43 |
Refinement procedure
| Resolution | 30.000 - 1.400 |
| Rwork | 0.196 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bex |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.031 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 31077 | |
| <I/σ(I)> | 17.4 | 1.8 |
| Completeness [%] | 66.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 20% PEG molecular weight 4000, 100 mM LiNO3 and 100 mM imidazole pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
