2FNS
Crystal structure of wild-type inactive (D25N) HIV-1 protease complexed with wild-type HIV-1 NC-p1 substrate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-12-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.060, 57.369, 61.302 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.000 - 1.850 |
| R-factor | 0.19968 |
| Rwork | 0.195 |
| R-free | 0.24308 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t3r |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.034 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.000 |
| High resolution limit [Å] | 1.850 |
| Rmerge | 0.056 |
| Number of reflections | 14812 |
| <I/σ(I)> | 8.5 |
| Completeness [%] | 92.7 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 126mM Sodium phosphate pH 6.2; 63mM sodium citrate; ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
